| 3x SDS loading
buffer |
94ul |
| 2-Mercaptoethanol
(nacalai, 21418-42) |
6ul |
| Total |
100ul |
| 0.5M Tris-HCl (pH
6.8) |
6ml |
| DL-Dithiothreitol
(Sigma, D9779) (4℃保存) |
0.9255g |
| SDS |
1.2g |
| BPB |
60mg |
| glycerol |
6ml |
| MilliQ |
up
to 20ml |
| Total |
20ml |
| 終
濃度 |
||
| 1M Tris-HCl (pH
6.8) |
6ml |
150mM |
| (+-)-Dithiothreitol
(Wako, 048-29224) |
1.851g |
300mM |
| SDS |
2.4g |
6% |
| BPB |
120mg |
0.3% |
| glycerol |
12ml |
30% |
| MilliQ |
up
to 40ml |
|
| Total |
40ml |