薬剤誘導性Cat/Sod1共発現

実験1
　細胞：3T3L1-TetON-mCatalase/mSod1 (Day5, 35%分化)
　処理条件
　　・HX/XO：通常培地, over night→DCFDA染色→0-2.4mM hypoxanthine+0-40mU/ml xanthine oxidase in DMEM(PhenolRed-), 30min
　　・HX/XO+Dox：10ug/ml Doxycycline, over night→DCFDA染色→0-2.4mM hypoxanthine+0-40mU/ml xanthine oxidase+10ug/ml Doxycycline in DMEM(PhenolRed-), 30min


実験2
　細胞：3T3L1-TetON-mCatalase/mSod1 (Day5, 40%分化)
　処理条件
　　・Dox：10ug/ml Doxycycline, over night→DCFDA染色→10ug/ml Doxycycline in DMEM(PhenolRed-)(nacalai, 08489-45), 30-180min
　　・HX/XO：通常培地, over night→DCFDA染色→0.6mM hypoxanthine+10mU/ml xanthine oxidase in DMEM(PhenolRed-), 30-180min
　　・HX/XO+Dox：10ug/ml Doxycycline, over night→DCFDA染色→0.6mM hypoxanthine+10mU/ml xanthine oxidase+10ug/ml Doxycycline in DMEM(PhenolRed-), 30-180min


実験3
　細胞：3T3L1-TetON-mCatalase/mSod1 (Day5, 30%分化)
　処理条件
　　・Dox：10ug/ml Doxycycline, 21hr→DCFDA染色→10ug/ml Doxycycline in DMEM(PhenolRed-)(nacalai, 08489-45), 1-2hr
　　・HX/XO：通常培地, 21hr→DCFDA染色→0.6mM hypoxanthine+10mU/ml xanthine oxidase in DMEM(PhenolRed-), 1-2hr
　　・HX/XO+Dox：10ug/ml Doxycycline, 21hr→DCFDA染色→0.6mM hypoxanthine+10mU/ml xanthine oxidase+10ug/ml Doxycycline in DMEM(PhenolRed-), 1-2hr
　　・NAC：通常培地, 21hr→DCFDA染色→10mM NAC in DMEM(PhenolRed-), 1-2hr
　　・TBHP：通常培地, 21hr→DCFDA染色→50uM TBHP in DMEM(PhenolRed-), 1-2hr