〇CHO　Armc5 CRISPR　設計3　ウェスタンブロット

細胞：2.5 x 10^6/6cm→翌日、100% confluent
タンパク濃度：3.3-4.3ug/ul
抗体
　・anti-ARMC5 (Novus, NBP1-94024, F118163), 1/1000 + anti-mouse Ig (NA931V), 1/2000, Skim milk
　・anti-RPB1 (Santa Cruz, sc-55492), 1/200 + anti-rabbit Ig (NA934VS), 1/2000, Skim milk
　・anti-β-actin (Sigma, A5441), 1/5000 + anti-mouse Ig (NA931V), 1/2000, Skim milk
　・anti-SREBP1 (Santa Cruz, sc-13551, H2024), 1/200 + anti-mouse Ig (NA931V), 1/2000, Skim milk
　・anti-SREBP2 (Merck, MABS1988, 4228985), 1/1000 + anti-rabbit Ig (NA934VS), 1/2000, Can Get Signal
　・anti-SCAP (Santa Cruz, sc-13553, L1622), 1/200 + anti-mouse Ig (NA931V), 1/2000, Skim milk
　・anti-INSIG1 (Santa Cruz, sc-390504, C1224), 1/200 + anti-mouse Ig (NA931V), 1/2000, Skim milk
　・anti-INSIG2 (Proteintech, 24766-1-AP, 00071240), 1/1000 + anti-rabbit Ig (NA934VS), 1/2000, Skim milk