CHO　Armc5 CRISPR　設計3　mutant allele

〇CHO　Armc5 CRISPR　設計3　mutant allele

ATGGCTGTTTTGATGGTGAATGAGGTCAGAGAGGCTGGCTACAGTCAGCCCACAATTCCGGCACCTGAAAGCTTTGTGGACTGCAGGATCATTGGTCTTGGCAAAGGTTTCTTCCCCAGGGCCAGAGCCTGACCTACATAGGTACTGCTGGCCTAGCAACTTTCCCCCGGCGGCTGGTTGCTCCGGCAGCGCCAGGAGAGGGCGCTCATGCAACTCAGCCTCGCGATCGAGGGACGACTTGAGGGCTCCCGGGAATTTTGATGCCCGGTTCCCCAGCAGCTTCGCCCCATCTGGATTCTCCCTAGCAGCTTCTGGCGCCGCAAGTTCCATTGCGGGCGAAGAGCGCTCGCCACCCGTTTAGCTCGTCAGCACTGGGGGCTTGGAGGCCACCACCGGAAACGGAAAGGTCTACAAGCCCGAGCAGCTCTAGGAAGCAGCAGCGAACTTTCTCGGTAGGTTTAACTCTCTCATTCACTGAACGAGAGCTTGGAGTTCATCAGTCCGGCTTCGGGACCCTAGACCACCACGCTTGGGGGTTGTCAATAAAATCTGAATGGGGAAAGGCGGTCGTTGACAAGGACGTACCGGCAGCGAAGTAGCTAAGTCACGTTGAGCAGCACAGACTCCACAGCGGGCCGCCTGGTTCTAGAAGCAAGAGCTCACCCTTAAGCCCTGGGCGGAGTTTCTCACGCACCTCAAACTGCTCTAAGCTTGGGCTTGACTACATATCCCAGCATGCACCGCACGCGCCTCTTACTGTCACACAGATAAATACCCTTCTCGCCATGTCTTCTGAGACTGTGCTAGTACCCAAGTTCCAGAATCTAGAACTTCTACTTCCGACTCGCATGATAACTGCGGCGGCGCTATTCAGAGGAGCAGCCTGGCCACGCCCCTCTCTTCCAGTTCCGCTCTCTCAGAGAACCGGCGAGACGCGGGGCGGAGCCTTAGCCCCGGGCCAAGATGGCGGCTGCGAGACCGGCCCTTACGGACTCTCTCTCGTTCTGCCTCGCGCAGCTCACGGCGGCGGCCGGGGAGGGGCCGGGTTGGGGAAAGGACCCAGCTACCAACGAGACACCCCTGGGCCGCGCGCTCTTAGCCCTCCGCACGCGCCACATCAAGGCAGCAGACGGAATTGAGCGCTTCCGGGCACGCGGCGGGCTCCGCCCCCTTCTCGCCTTGCTACGCCGAACGGCTGCTGCGGGCCCCGCCCCGTCCCATGCAGGCTCTGGCTCCGCCCCTTCGTCGGTTGCGTCAGCTGTTTCTTCCCCTGGCCCCGCCCCCGCTCCTGAGTCGCCTTTGACACCTTCGTCGCCAGTGCGCCTGCGCAAGACGCTGGATTTGGCGCTCAGCATTCTAGCCAACTGCTGTACGGAAGAGGCGTGCCGAGCCGAAGTGCGTAGACTTGGAGGCATTCTCCCTTTGGGTAAGTGCTCCGCCCCATTGCTTAGAAAGGTTCCGCCCACAGGAGCTGTGCAATCCTTAGTTCTTTATGTCACGCCTCTGAGTGCGCGCTTTGTGATGGTGCCGCGCCACTCAACCTTCTCAGGGGTCATGCTGTGTGACGTCACTGCTCCCCATAAATTATACCCGGACCCGCCCAGACCAGGATTAGGTATTGGCTCCTGCCCCACTTTTGAGCGAATGGTTTGCACTTTCTACTTTTGGTGTTACAGCGGACAGTGCTTACTGCTGTGTCCGTCCCTGTGCAAGGCTTCACGTGGATGAGCTGGAGGTCAGAAAAGTGTAGTTTTAAAAGTTCGGCGAATGGACTTAGAAAGTGGTGCAGTCAGACATAGCGGGCAAATCACCCAGCCCCTCCAAAACTAGAATCCAAGATTGAAGTGGGCATAAAATGAAATTCAAATATAAAATGCCCAGACTCCAAGCTCAGGCTGGGTACTTAGAAACACACAGTAAACAGTAAATTACAGAAAAGGATAAGCCTTTCTGTGTATTCGCTCATTTGGCGCCTCCTAATGGTGATGATGGGAACAAAGTTGTGTCATTGCTTTTCCTCTGATAGTATGCCAGTTTTGACaaaaaaacaaaaacaaaaaacaaaaaaaaaCTTAATTTTTTTTTTTTGgtttttcgagacaaggtttctctgtattgctttggagtctgtcctggaacttgctttgtagaccaggctggccttgaactcacagagatccacctgcttttgcctcccaagtgctgggattaaagatgtgagccaccacCTAAAAATCTTAATTTTTAAAGTGAAGCATTTATATATTTAAAACTTGCATAGAATGCACTTGTACAATACTGTGTTTGCCAAGAGGCTTGACAATAGCCCCTGTAAATACCAAGTTCTCTCTAACCTTGCTTCCACATTTCTGTGCTGGGCAGGATTACCTTTTGTTTGTCAGAAATGAAAGCTGACTTGGTTCAAGCCAAGTTACCTTCACTACTGTGACAACTTCAGCCATTCTTCTGGCCCCATCCTTGTCTTGTAATACCCCCATCACTACAGTTGCCAGAGTGTATAGGTCACTTCCACTCTTAAAACCCTTTTGTGGCTGCTGTGCCCTCTCATGTAAATTTTGACTCTCGGACAGTTATTTGAGGGACTATGTGAAATTACTGAACTGTTGTGACACTATTACCGTGGGGCATTTAGAAACGCTGCTTTCAAACCTGAGGCACGCTAGCCTTTCCATTTACCATCCTTCTGGCAAATATCCATTCAGTCCTCTAACTCTTGCTCACTGTTTAGCATCTTCTAAAGATTCTTCCCTAACCTTCTCAAATCATGTTTGTTTCCTCCTCTCTCATACCTCTCTCTCCCTCAGAGTTTTCTTGTCCTGACTACCTGGTCCCAAATAAACGCACAAAAGCTTACATTAATTACAAATGCTCGGTCGATAGCTCAGGTTTATTATTAATTAGCTCTTATATTTAAGTTAACCCATTTTTATAAATCTATGTATTGCCACATGGCTTGTGGCTTTACGTGTCCTCTAGCATCTTGTTTCCCTTGTGACTGGCTTGGGTAGCTCCCTGACTCCGCCATTCTTCTTCCCTTCCTAATTTGGTTTTCCTAGTTCAGTTTTCCACCTAACCTTTTCTTGCCTGGCTGTGGTTAGTCAGCCTCTTTATTAAACCAATCAGAGCATAATTTACAGTGTACAGGAATATTCCATAGCACTCCTCCCTCTCTTTTTGGATTGTTGGGGATTGTGTCGACAAAGCTGAGGCGTTCTCGAAATCAGCTCTTGTTCGGTCGGCATCTACTCTATTCCTTTGCCCTCGGACGAGTGCTGGGGCGTCGGTTTCCACTATCGGCGAGTACTTCTACACAGCCATCGGTCCAGACGGCCGCGCTTCTGCGGGCGATTTGTGTACGCCCGACAGTCCCGGCTCCGGATCGGACGATTGCGTCGCATCGACCCTGCGCCCAAGCTGCATCATCGAAATTGCCGTCAACCAAGCTCTGATAGAGTTGGTCAAGACCAATGCGGAGCATATACGCCCGGAGCCGCGGCGATCCTGCAAGCTCCGGATGCCTCCGCTCGAAGTAGCGCGTCTGCTGCTCCATACAAGCCAACCACGGCCTCCAGAAGAAGATGTTGGCGACCTCGTATTGGGAATCCCCGAACATCGCCTCGCTCCAGTCAATGACCGCTGTTATGCGGCCATTGTCCGTCAGGACATTGTTGGAGCCGAAATCCGCGTGCACGAGGTGCCGGACTTCGGGGCAGTCCTCGGCCCAAAGCATCAGCTCATCGAGAGCCTGCGCGACGGACGCACTGACGGTGTCGTCCATCACAGTTTGCCAGTGATACACATGGGGATCAGCAATCGCGCATATGAAATCACGCCATGTAGTGTATTGACCGATTCCTTGCGGTCCGAATGGGCCGAACCCGCTCGTCTGGCTAAGATCGGCCGCAGCGATCGCATCCATGGCCTCCGCGACCGGCTGCAGAACAGCGGGCAGTTCGGTTTCAGGCAGGTCTTGCAACGTGACACCCTGTGCACGGCGGGAGATGCAATAGGTCAGGCTCTCGCTGAACTCCCCAATGTCAAGCACTTCCGGAATCGGGAGCGCGGCCGATGCAAAGTGCCGATAAACATAACGATCTTTGTAGAAACCATCGGCGCAGCTATTTACCCGCAGGACATATCCACGCCCTCCTACATCGAAGCTGAAAGCACGAGATTCTTCGCCCTCCGAGAGCTGCATCAGGTCGGAGACGCTGTCGAACTTTTCGATCAGAAACTTCTCGACAGACGTCGCGGTGAGTTCAGGCTTTTTCATGGTGGCGGGATGCAGGTCGAAAGGCCCGGAGATGAGGAAGAGGAGAACAGCGCGGCAGACGTGCGCTTTTGAAGCGTGCAGAATGCCGGGCCTCCGGAGGACCTTCGGGCGCCCGCCCCGCCCCTGAGCCCGCCCCTGAGCCCGCCCCCGGACCCACCCCTTCCCAGCCTCTGAGCCCAGAAAGCGAAGGAGCAAAGCTGCTATTGGCCGCTGCCCCAAAGGCCTACCCGCTTCCATTGCTCAGCGGTGCTGTCCATCTGCACGAGACTAGTGAGACGTGCTACTTCCATTTGTCACGTCCTGCACGACGCGAGCTGCGGGGCGGGGGGGAACTTCCTGACTAGGGGAGGAGTAGAAGGTGGCGCGAAGGGGCCACCAAAGAACGGAGCCGGTTGGCGCCTACCGGTGGATGTGGAATGTGTGCGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAAAGCGCCTCCCCTACCCGGTAGAATTC TGTGCTGAGCAGCTGAGTCTGGGTGGGGGACTGGGCCCACTTGTCAGCCTGGCTTCTCACCCTAAACGGCCAATACGTGAGGCAGCCATCCTGATCCTTGCCAATCTGTGTGCTCAGGGCCTGGTGCGGCCTGCACTGGGCAATGCTGGTGGTGTGGAGGTGTTACTAGGTGAGCTTAGGCGACGACGGGGTTCCAGCGGGACCAGCTCAGCTGCCCAGCAGCCCCTAGTGCGGGCTGTGTGTCTCTTGTGCCGGGAAGCCATCAACCGGGCCCGGCTTCGGGATGCTGGTGGTTTGGAGCTGCTGATGGGCCTCTTGCAGGACCCTGGTGCCAGTGCCTGGCACCCACGTGTTGTGGCTGCTCTTGTAGGCTTCCTTTATGATACTGGGGCCTTGGGCAAGTTACAGGCTCTGGGCCTCGTGCCTCTTCTGGCCAGGCAGCTATGTGGTGAAGCTGGTGAAGAGGAAGAGGAAGGAATAGAAGCTGCTTCCTGGGACTTCCCTGAGGAACGGACTCCTGGGCAGACAGAGGTGGGAAGCTTCCGAAGCCTTAGGTGAGTCCTCTTGCCAGGGCCTGAGGGACAGATGGTTCTTTGGGGAGGGAGTTTGTGTCTGCTGTGGTCATCTTCCTTACCTCTGCCTCTTTTCTGTCTTGAGATTTACCAAGGTTTATTCCTAATTCATTGTTCTTCCCTAAAATGACTTGACTCTGCCATCTGACCCTGGCTCCACCCCATCATCCAGAACTCTGGGTCCTACTCAAGTTGATTGGGGATAGCATGTGACCTGGTTACAGTGGTATGCTATGGAGCCTGAtttttatttatttgtatttttttttttttGAGACGGCATTTTACTTTGAAGGCCAGGTTGCCTGACtctcattggatagcgcaggctgtccttgaacttgcagctatcctcctgcctGTTGCTATTCTGTTACCATCACCGAgacatttgggaaactaattggcttttctgagccactgatttcttgtgtgtgcaatagagggtaacagcatacatacccacagagttgctgcaaagactGCCCTTTACCTCGCTCCGAGTGACGGTCAGTAATGGCAGTCTTGTGGactcttccactctcactccctgaaggcctcctctgtagttcctaaacatgatgagaaagtaccgtctgagagtctctctgcctagaacactcttcctcctgggtgccttcatctctcacctccttcaagtctttgtttggttctaccatctttataaggaggcgtgatcaccatcctatgtagtatttcacctctcctctggtcttcagtgtcctctccccctgtctgttaccataccttgttacactgacttcacagcaGCATACCATGAAAGGGTTGCTTTATTTTGTTTGGTTTGGACAAGGGGTctgatcttaaacttatcatcctcctgcatctgtgcctgagtgccaggatcacaggcataaaacactgtgTAACTAGTTATACCATGTAgtttacatgctgtttctcctcagtgggtcataaactccaggaataacatgttttgttcattgcttttcttgagcagctgaagctggatggcacacggtaAAAACACTAGTAATTCAGAACAAGGTGTGGTCCACCTCAGCTTTGTGTCTTCCCCACTCACACTCCTTCACAGAACTGTCccttctttcctttctctccccctccttccttcctttctctttcttccctctctccttcccatctgtctacttttctttctttTTGAGACAGCAGCAGGTTGTCCTGGAACTCACTGCCCCGGCTTGCCTTGAATTTGTGGCACAGAAATTTTTGGTTCTGCGTCCTCACCTTGGCTGTGGGTTCAGACCCCTTTTCTCCTTCTCCTCCGTAGGTTGTGGCTGATTTCTGAGGGTTATGCAGCAGGCCCTGGAGATATCTCCCCAGATTGGTCTCCTGAGCGTTGTCCGATGCCAGAGCTATCCGAGTCAGTGAGTCCTACCCCTGGCCAGACCGCAGCGTCGACACCCCGAACACTTCGCAAACTGGGCCGTATCCCTGCTGCCACTACTGAGGAGCCTTGGGGCCAAGAGGGGCCAGCACTGTTGCTGTTGTCACGCTTCTCCCAGGCTCCTGACCCAAGTGGAGCTCTAGTGACTGGTCCAGCACTTTGTGGCCTGTTGGCCTATGTTACAGGTGCACCAGGACCACCAAACCCACGTGCACTACGTATCTTGGCACGTCTCACCTGTAACCCTGCCTGCCTTGAAGCCTTTGTGCGCAGCTATGGTGCAGCACTGCTGCGCGCCTGGCTGATACTCGGTGTCTCACCAGATGACTGGCCTGTGCCACATGCCCGGCCTGTACACCGAAACCAGCACCGAGAGCTGGGTATGTGCCCCTATCTCTATCCATATGAAAGGCCTGTTTTCCTTCATACGCTTTGTGGTCCTAGAACCTCTATTTCCTACTCACTTGCTCTCACAGTTCTCTCCAGCCCACCATCCAAGCTTCTCAGCCTCCAGGCATGGGGGTGGGGAGGTGACTGGAAGGCTGGGGACTATCCCTTCTGTTCCATAGCCCTGGAATACAGAGAGTTGTTCTGTCCTTTGCCCAAATAAATCTTGGGGCCCAAATAACCTGGTACCAGGTTTGGCTCCTATTACCGTAGAATCTGACCTTTCTGTGTTTGTTTACCCGCTCTACTCTTGTTCTGTACTGTCTCATTTCTACCTGGGCACACTCACTTATGTGCTCAGACCCATCCACGGTGTCCTCTCAGCCTCTGCTACCATTTTCATCTTGGGACTGCCTTGTCTTCTGGGCCTGACTGTAGGCTGTTTCATTTCTTAAGGGCCCAGATCTGCACCCTGACCATCCATTTGCCTGTCCTTGACCAGTGCTCTTTGTACTCCTCTGTTGATTTCTTGCCCTGAGATCTTACTCTCCTCCCATTTACAGGCCCAGAGTCCTGTCTTGCCCACGACACCCCAACCTGACCCTTGTGTCTGTCTCTGCAGGTGAGATGCTGCTGCAGAACCTGACTGTTCAGGCTGAGTCCCCTTTTGGAGTGGGTGCCCTCACCCACTTGCTGCTCTCTGGGAGTCCCGAGGACCGTGTAGCCTGTGCACTGACCCTACCCTTCATCTGCCGGTAAGTGGGAAGCGGGTGTCTTGCAGGGACTGTGAATAAGATGCCAGCTCTCTGAGACCCCTGCTGAAAGATGCCCTTTTCCCCACAGGAAGCCTACTCTGTGGCGCCGCTTACTTCTGGACCAGGGTGGCCTCCGCCACCTCCTCACAGCACTAACTCAGCCGGTTCCACACCCACTCTTCCTCTTCTTTGCAGCAGATTCCCTTTCCTGTCTCCATGAGCTGGTGTCTCCCACCGTGAGCCCCATCCTTTTCTCTGTCATGCCCTCGGAACCTGACTCAACCTCCCCTTGCCTCTATGAACCTCTCCTGGGCCCAGCCCCTGTCCTAGCCCCTGACCTGCACTTCGTTCTGGATTCAGGCCTACAGCTCCCTGCTCAGCGGGCTGCTTCAGCTACTGCCTCCCCCTTCTTCCGGGCTCTGCTATCCGGAAGCTTTGCTGAAGCGCAGATGGATTTGGTACCACTTCGAGGCCTGTCCCCTAGTGCAGCATGGCCAGTCCTGCATCATTTGCATGGCTGTCGGGGGTGTGGGGCTGCCCTGGGGTCAATACCCCCACTTGGCCAGCCCCTGCTGGGATCCAAGGCTGAGGAAGCTCTGGAGGCTGCTGGTCGTTTCTTGCTGCCTGCACTAGAGGAGGAGCTAGAAGAGGCTGTTGGCCGCATACACTTGGGTCCCCAAGGTGGCCCTGAGTCAGTGGGTGAGGTATTCCGATTAGGTCGGCCCCGGCTGGCTGCCCACTGTGTACGATGGACACTGGGGCCAGGGCAGTGCCCTAGAAAACGGGCCCTGGCCCTTATAGGGCTTGTGGAAGCAGCAGGTGAGGAGGCAGGGCCTCTCACTGAAGCTTTGCTGGCTGTGTTAACGGGAATTGAGCCATAGCACAGGTGCTCCAGGCTAAACTGTTGGCATCCCTGGAAGAGCACCCAGAGATGAGTTCGTTGGACGGGAGGCTTCTCTCAGCATGGCATGAGGGGAGACTGCGAAGGAGCTATCCTCAGGGACTGAAGAGAAAGTGGAACCAAATCCAGGCTGAAGATCTGAAGATCTGTGACTAAAAGTCAGCACCAGAGTCTGTGCATGGTGTCCAAGAGGACAGCTCAGGGCCCCAGGATTTGGTCTGACCTGGCTCTGGGGTATTAAAATTAAAAATGAGATTTGGACACTGCTGTTGTATGGTGCTTCTTTCTGCTTGCTTTCCTCTGGGGATACTCTACCCATTCACCTGCCAAGAGACCTATGCTTTGACATGGG