~ƒŠƒAƒ‹ƒ^ƒCƒ€PCRƒvƒ‰ƒCƒ}[ƒŠƒXƒg

‘•Œψ—¦/R2/ƒAƒj[ƒŠƒ“ƒO‰·“xor extension time/ŽŽs‰ρ”





LightCycler 96
Light Cycler 7900HT

Žν
ˆβ“`Žq
exon junction
•β‘«
‘•’·
Essential
SYBR
Thunderbird
Thunderbird Next
TB Green
SYBR
Thunderbird
Thunderbird
Forward Reverse
human
ABCA1





84.2(1)/0.98(1)/60





GCACTGAGGAAGATGCTGAAA
AGTTCCTGGAAGGTCTTGTTCAC
ABCG1

variant 2-7/6



98.1(1)/0.94(1)/60





GTTTGTGGGTGTCTCGTGCTC
CACCAATCTGCCTACATCTTCCTAG
ACACA

variant 1-5


104.2(1)/0.99(1)/60


97.7/0.82/1



CTTAAGGTGACTCGACAGTC
CTGCCATCATAGGACAAGAG
ACLY

variant 1-4


99.7/1/1
103.9/1/1

87.6/1/2



CTGGGAAGGAGTATGGGGTTC
CAGTAGGGTTCCTGGCTCTGTTAC
ARMC5
variant 1-4/4 81
85.1/0.97/1

98.4/0.956/8 109/0.98/1
95.2/0.98/2



GTACGGCCTGCTGACCTATG CAGGTGAGGCGTGACAGAATG




90.9/0.97/3





CTGCATCATTTGCATGGTTGTC
CTCTTCCAGCTCCTCCTCCAG

variant 1-4/4
PX458-hARMC5-1—p
265 82.4/0.94/1







AAAGGACCCAGCGACCAAC ACTAGACGACGACACAGCGG

variant 1-4/4
PX458-hARMC5-2—p
147 116.8/0.96/1







CTACTCGCGCTGCTACGG
CCNA2

all

99/1/1







AAGAAACAGCCAGACATCACTAACAG
GCACTGACATGGAAGACAGGAAC
CCNB1

all

99.2/1/1







CCAGAACCTGAGCCAGAACC
TGGAGAGGCAGTATCAACCAAAATAG
CCND1



98.8/1/1







TTATTGCGCTGCTACCGTTGA
AACTGATCCTCCAATAGCAGCAAAC
CCND2

all

101.1/1/1







GCCTCCAAACTCAAAGAGACCAG
TCAACTTCCCCAGCACCAC
CCNE1

all (v1,3-5)

102.1/1/1

81.8/0.99/1





GAGAACTGTGTCAAGTGGATGGTTC
GCTGTCTCTGTGGGTCTGTATGTTG
CYP11B1

variant 1,2/2



82.9(1)/0.80(1)/60





TGCGTGAACCTGCCTTG
ACTCTCAAACTGGGTGCTTCCTC




104.4/0.96/1

76.3/0.92/1



ACGCCATAGACTGGGTTGCTG
GAACCTGCTGACACCTTGATCTTAG
CYP11B2

P450aldo



87.9(1)/0.97(1)/60





CGAGTGTTGGGTCCTCTGCTTC
GCCCAATCCTGTCTCTCCTGTTG




110.3/0.97/1

82.3/0.89/1



TCCTAGCCCAGCAGAGAGAGTTG
CCCAGGAGATAAAGATGATGTGG
CYP17A1





96/0.987/3





CGTAACCGTCTCCTCCTGCTG
TGATAACTTCTGTGCCCTTGTCC
CYP21A2

variant 1-4



107.1/0.99/3





CGTTGTGCCCTTAGCCTTG
CAGAACTCATGTGGCCTCTCC
FASN




83.4(1)/0.97(1)/60
95.6(1)/0.99(1)/60

101.1/0.97/1



CACCCAAGGCCAAGTACCATG
GATACTTTCCCGTCGCATACC
FKBP5





95.2/0.98/1



Z103.3

AGCTATTCCAAGACTCAGATGATGC CCCTCCCCACACACACTTTT
HMGCR

variant 1,3/3

94.6/1/1
90.1/0.97/1
95.6/0.99/3

98.1/0.985/2



CTGCAGAGCAATAGGTCTTG
GACGTGCAAATCTGCTAGTG
HMGCS1

variant 1-9/9


96.0/1/1
98.4/0.995/4

92/0.995/2



CATACAGTGCTACCTCAGTG
TTCAGCAACATCCGAGCTAG
HSD3B2

variant 1+2



99.2/1/2
83.1/1/1




AGTGGGTTGGTTCCCTTGTG
CAGGTGGGTGGAGTTTGATG
LDLR

variant 1,2/5


~






AACCGCTGCATTCCTCAGTT
TCTGAGTCACAGACGAACTG

variant 1-4,6/5

102.9/1/1

92.1/0.995/2

94.6/1/1



TGTTTTCTGTCGTGTGTGTTGG
GTCAACCTGCCCCTCTCTGTC
NR1H2 (LXRƒΐ)

variant 1,2/2



106.4(1)/1.00(1)/60





TATCGGCTCTCATCCCTTGG
ACCCTTTCACCCCTTCTGG
NR1H3 (LXRƒΏ)

variant 1-6/6



101.1(1)/0.99(1)/60





TGATGCCGAGTTTGCCTTG
ATGGGGATGGTGGATGGAG
RPLP0

all (v1,2)
143
89/0.96/2
93.8/1/1
92.7/0.978/7
89.7/1/1
106.9/1/3



GCTCCAAGCAGATGCAGCA
CCGGATATGAGGCAGCAG




98.1/1/2





TGATCAAGACTGGAGACAAAGTGG
ATGACCAGCCCAAAGGAGAAG
SCD




96.1/1/1


80.5/0.98/1



AACTTGATACGTCCGTGTGTCC
AGTTATCCACCGCTTCTCCAATG
SOD1


165





81.9-89.2


TGGCCGATGTGTCTATTGAA TTACACCACAAGCCAAACGA
SREBF1

all (v1-13)
144

80.0(1)/0.96(1)/65
85.9(1)/0.96(1)/60
91.2/0.972/4

107/0.985/2



GATGTTTGTTTGTTAGTGGGAGGAG
TGGGAGGTGAGAAGGGACAAC
Z
all (v1-13)









GTGCTGCAGGAGTCTGAGAGA GCTGGACCAGACTCTGCCTT
SREBF2





91.9/0.993/4

92.1/0.993/3



GGGGCTGGGAGAAATGAAG
GTGGAGGTAGGAGATGGGGTAG
STAR




98.1(1)/1.00(1)/60
103.1/1/1
84/1/1




GGGCCTGAGAAGTCTTGCTTTATG
TGCTTGTTCTGTGGTGTTGCTG
mouse
Acaca

Acc1
186





’ 85.8-88.2


GAGGTACCGAAGTGGCATCC GTGACCTGAGCGTGGGAGAA

84

Z102.9% (1‰ρ)
100.1(3)/1(3)/60


Z 92.9-97.1
Z96.3

AACTGGCCTTCTTGATGTTAGGAG AGCACCGAGACTGAACTGTAAGG
Acly

variant 1+2
65
Z93.2% (1‰ρ)
102.9(3)/1(3)/60


Z 85.3-98.5 Z
Z106.7ACCCTTTCACTGGGGATCACA GACAGGGATCAGGATTTCCTTG
Adgre1
F4/80






77.6 Z93.2-96.8
TTACGATGGAATTCTCCTTGTATATCAT CACAGCAGGAAGGTGGCTATG
Adipoq







72.9-81.4


GATGGCAGAGATGGCACTCC CTTGCCAGTGCTGCCGTCAT



Z 93.5%







CAAGGCCGTTCTCTTCACCTAC TCCCCATCCCCATACACCT
Akr1b7









Z79.0

AAGCTCTGGGCATCTCCAAC ATAAGGGTGGCTCTCAATCTGG
Arg1


102





85.8-93.0


GGCAACCTGTGTCCTTTCTC ACACGATGTCTTTGGCAGAT
Armc5



Z 83%




~
Z91.2

TGTGGCGCCGCTTACTTC AGAAGAGGAAGAGTGGGTGTGG




98.3(1)/0.98(1)/65
100.3(3)/0.997(3)/60


Z 83.5-91.5


TCCTGACCTGCACTTCGTTCTG AGCGGCACCAAATCCATC
Ccdc3


124





Z 88.7


TGGTCCAGGACTACTCTTATTTCTT GAAAACATCCTTCTGTTCTCCTG
Ccl2







Z 88.8-95.1
Z98.5-100.8
CCACTCACCTGCTGCTACTCAT TGGTGATCCTCTTGTAGCTCTCC
Cd36










AAGAACAGCAGCAAAATCAAGG AGACAGTGAAGGCTCAAAGATGG
Cebpa










TGCCAAACTGAGACTCTTCACTAAC CCCAAACATCCCTAAACCAAAA
Clec4e


126








CCAATTTCATGTGCTCCTCTTTG CAGGTGGAGTGTGGGATGTG
Col1a1









Z92.8

GTGCTCCTGGTATTGCTGGT GGCTCCTCGTTTTCCTTCTT
Col3a1











GGGTTTCCCTGGTCCTAAAG GGAGAAAATGGGAAACCAGG
Col6a3

variant 1+2
124





Z 90.5
Z95.7

CACCTCTTCAGGCAGCACAC TCCACACAAGTCCCAGCATC
Cox4i1










TGATTGGCAAGAGAGCCATTTC AGCGTAAGTGGGGAAAGCATAG
Cul3

variant 1+2


85.2(1)/0.99(1)/65






GTTCTTGCAGCTGTGTTTTAGAAAG
AGCACAGAGGAACGGACACAC
Cxcl12











CAAGGTCGTCGCCGTGCTG CGTTGGCTCTGGCGATGTGG
Cybb

gp91phox
204





Z 93.7


TTGGGTCAGCACTGGCTCTG TGGCGGTGTGCAGTGCTATC
Ddit3

chop
variant 1+2
511








AGTCATGGCAGCTGAGTC TCATGCTTGGTGCAGG
Dgat1


199








ACCGCGAGTTCTACA AGGGGAACGCTCACTAGGTA
Dpp4

variant 1+2
184




91.9

GTGGCAAGAGGGGATCACTA CCCAGCCTGTGGTACTCATT
Elovl6

110




Z 88.0-92.0

GAAAAGCAGTTCAACGAGAACG TATCTTTGGTGGTCGGCATCT
Fabp4










CCGCAGACGACAGGA CTCATGCCCTTTCATAAACT
Fasn







’ 87.4-94.2


GAGAAGCCATGTGGGGAAGATTTC TGAGCAGGGACAGGACAAGAC


108

Z106.9% (2‰ρ)
101.7(1)/1(1)/60





CATCCACTCAGGTTCAGGTG
AGGTATGCTCGCTTCTCTGC
Fgf2









Z97.1-97.5

AACGGCGGCTTCTTCCTG CACAACTCCTCTCTCTTCTGCTTG
Foxp3

variant 1+2+3
89








CCCAGGAAAGACAGCAACCTT TTCTCACAACCAGGCCACTTG
G6pc


228





Z


TTACCAAGACTCCCAGGACTG GAGCTGTTGCTGTAGTAGTCG
Gclc










TCCTGGAGGAAGGCATCGAT CGGAACTCTACTCTCCATCC

exon 2


94.9/0.975/1

102.5/0.99/1
95.9

CATGTTGGTGTCCTTCGATCATG TGGTTGGGGTTTGTCCTCTC
Gdf11











TCTTCCCATGAACATCACACCTTG ACCCTGACTGCCCTTCTCTTC
Gys2


135





Z


TAGAGCCCACATCACCACCAAC TCTCATCCTCATCTTCTTCGTCTTC
Hmox1


101








GTCACTTCGTCAGAGGCCTGCTA GCGGTGTCTGGGATGAGCTAGT
Hsd11b1

variant 1+2
159





Z 84.4


GCTCACTACATTGCTGGCACTATGG TCTTCGCACAGAGTGGATGTCGTCA
Hspa5

Grp78
variant 1+2









ACCTATTCCTGCGTCGGTGT GCATCGAAGACCGTGTTCTC
Il6










ACAACCACGGCCTTCCCTACTT CACGATTTCCCAGAGAACATGTG
Insig1

all (v1)

100.5/1/2







TGCCCAAAGGAGGGAGGTTG
TCCTCCAACTCACTGCTGCTC
Insig2
~
all (v1-14)

97.4/1/2







ATAGCTGGCCGTTCCCTCTC CACATGTGGGTGCTGTGACG
Kdm1a

variant 1+2
146





Z92.7-110.0
GGTATCTCGTTGGCGTGCTG GTCTTGGGATTGGCTGTGG
Lep










GATGGACCAGACTCTGGCAG AGAGTGAGGCTTCCAGGACG
Lipe

HSL
variant 1+2
213








CCGCTGACTTCCTGCAAGAG CTGGGTCTATGGCGAATCGG
Lpl











TGGGACTGAGGATGGCAAG GGCAGGGTGAAGGGAATGT








Z


CCCTGAAGACACAGCTGAGG GGGTGTACCCTAAGAGGTGG
Metrnl











ATGCCTTGGTGTGCCATTTAATAG TGCTCCATACATTTCCATCACAG
Mlxipl

ChREBPƒΐ








CGAGGTCCCAGGATCCGA TCCCGGCATAGCAACTTGAG

ChREBPƒΏ+ƒΐ 88






~
GCTCAACGCTGCCATCAAC GTCCCGCATCTGGTCAAAG

81




Z 95.4 Z100.5-103.3 ~
ATGTCGGTCTGTTTCCTCACAAC TCCTGCTCTGCCTCTCTGCTC
Nampt

155




83.9-86.3

GGCCACAAATTCCAGAGAACAG CCAAATGAGCAGATGCCCCTAT
Ncf1

p47phox
variant 1+2
212





Z 91.2


GATGTTCCCCATTGAGGCCG GTTTCAGGTCATCAGGCCGC
Nos2










CAGCTGGGCTGTACAAACCTT CATTGGAAGTGAAGCGTTTCG
Nqo1


101








CGCCTGAGCCCAGATATTGTG AGAATGGCTGGCACCCCAAA
Ostn










TGTGGACTTAGCATCACAGG AGCTGAGAGTCTGTCAAGG
Pparg

variant 1+2









ATCTTAACTGCCGGATCCACAA GCCCAAACCTGATGGCATT










TTAACTGCCGGATCCACAAAA TGAGACATCCCCACAGCAAG

218





’93.4


CCAGAGTCTGCTGATCTGCG GCCACCTCTTTGCTCTGCTC
Ppargc1a

Pgc1ƒΏ







Z93.9

CAGCCTCTTTGCCCAGATCT CCGCTAGCAAGTTTGCCTCA
Pck1


65





’


CCACAGCTGCTGCAGAACA GAAGGGTCGCATGGCAAA
Pdgfb









Z99.5

ATTCCACTCCAGCCAAGCAG GCCAGAACGTCATAATAGGCAAG
Plin2










GAGCTGGAGATGGAAGCAAAA GTGATAAGCCCGAGAGCAGAG
Pnpla2

Atgl
variant 1+2
193





Z83.6


GGTGACCATCTGCCTTCCAG TGCAGAAGAGACCCAGCAGT
Pygl


96





Z


AAGGGGTATGAGGCCAAAGAATATTAC TGGCTGATTGGGAGAAAAGAAG
Rplp0


100.3/1/2
97.8(2)/1.00(2)/65
99.5/0.99/4

93.3/1/1
Z83.5-108 Z94.6-102.7 Z100.7AAAGGAAGAGTCGGAGGAATCAG GGCTGACTTGGTTGCTTTGG
Scd1

150


105.7(3)/1(3)/60


Z89.5-102.1
Z101.7
Z101TGGGTTGGCTGCTTGTG GCGTGGGCAGGATGAAG
Serpine1

PAI1
193








TCAGCCCTTGCTTGCCTCAT GCATAGCCAGCACCGAGGA
Slc2a1

Glut1
207








CCATCCACCACACTCACCAC GCCCAGGATCAGCATCTCAA
Slc2a4

Glut4
101








GCGGATGCTATGGGTCCTTA GTCCGGCCTCTGGTTTCAG
Sod1


168





89.7


CAGCATGGGTTCCACGTCCA CACATTGGCCACACCGTCCT
Srebf1

variant 1+2

102.5/1/1
99.3(2)/0.99(1)/65
106.6(4)/0.998(4)/60


Z91.9-95.7

Z99.8-103.4TGCTCCTGTGTGATCTACTTCTTG TGGGGTCCATTGCTGGTAC
Tgfb1


95






Z101.1

ACCATGCCAACTTCTGTCTG CGGGTTGTGTTGGTTGTAGA
Th









Z110.3

CGAGAGGACAGCATTCCACAG CCAGAAAATCACGGGCAGAC
Timp1

variant 1







Z104.1-104.5

GTGGGAAATGCCGCAGAT GGGCATATCCACAGAGGCTTT
Tnf

variant 1+2






84.6-88.8


TGTGCTCAGAGCTTTCAACAAC GCCCATTTGAGTCCTTGATG








Z97.8
GGACAGTGACCTGGACTGTGG AGTGAATTCGGAAAGCCCATT
Ucp1


70





Z97.2-97.8
Z91.9

ATCAACTCTCTGCCAGGACAGT GTCCTTCCTTGGTGTACATGGA
Vegfa

variant 1







Z92.3-101.7

TCTCTTGGGTGCACTGGACC GTTACAGCAGCCTGCACAGC
Vegfb

variant 1+2







Z101.3

TGACGATGGCCTGGAATG GCATTCACATTGGCTGTGTTCTTC
Vegfc









Z96.4

AGTGAAGAAGTATGCCGCTGTGTC AAATGGTGGACTGAAAACTGGTATG
mouse/rat
Cat


198





Z89.9-103.7


CCAGCGACCAGATGAAGCAG CCACTCTCTCAGGAATCCGC
Rplp0


143





Z76.3-88.9
Z85.5

GCTCCAAGCAGATGCAGCA CCGGATGTGAGGCAGCAG
hamster
Armc5
Z


93.2/0.99/1







TGCCTTGAAGCCTTTGTGCG AGCAGCATCTCACCCAGCTC
Hmgcr
Z


102.2/1/1







GCCTGTCTGCAGATGCTAGG
TCAAGGACAACTCCCCAGCC
Hmgcs1
Z
all (X1, X2)

103.5/1/1







GATGCCACACCAGGATCTGC
ATCTGGCGCCACACATGTTC
Idi1
Z
all (X1, X2)

76.9/0.97/1







TTGGGAATACCCTTAGAAGAGGTTG
GGGATCCGGATTCAAGGTTACG
Ldlr
Z


99.7/1/1







TGAGATCACTGCAGGAGCCG
TGTCACCCTGGACTTGGTGG
Rplp0
Z
all (1,2)

97.4/1/2







CTGCTGGCCAATAAGGTGCC AGACCAGTGTTCTGGGCTGG
Sqle
Z


125.8/0.98/1







GTGGGGAAATGCCGAGGAAC
TGGTTCCTTCAGGTGCTCAGG
‚»‚Μ‘Ό rCat-rabbitƒΐglobin







91

ACTGACGTCCACCCTGACTA GCACCTGAGGAGTGAATTGG
FLPe








Z108.5
CCTACAGCTCCTGGGCAACGTGC CTGCTTCTTCCGATGATTCG